Environmental Biotechnology
Online EB edition > 2014 Volume 1 > Article


Methanogenic potential of biomass from roadside verges preserved with various additives
Cezary Purwin, Barbara Pysera, Maja Fijałkowska, Krzysztof Lipiński

Pages: 18-22

DOI: 10.14799/ebms227

open PDF file


The aim of the present research was to evaluate the chemical composition and storage capacities, as well as the efficiency and composition of biogas from biomass collected from roadside verges. The biomass was collected in July and October and then preserved in microsilos (10L) with and without formic acid, bacterial inoculant, bacterial‑enzymatic preparation, enzymatic preparation. After 180 days of storage, biomass samples were analyzed for chemical composition, organic dry matter (ODM) losses and biogas and methane yield (Oxi‑Top Control). Biomass from the summer period had a higher (p<0.01) content of dry matter, neutral detergent fiber, hemicellulose and cellulose and a lower (p<0.01) content of ether extract and acid detergent fiber. Loss of organic matter during preservation and biomass storage without additives was higher in the material from the summer period. However, when compared with the autumn period, summer biomass stored without additives had a higher methane production potential (288 vs. 215 LN CH4∙kg-1 ODM). The additive which most effectively reduced the loss of organic matter was formic acid. However, the most beneficial for biogas efficiency and methane were the bacterial‑enzymatic preparation (summer harvest) and addition of formic acid (autumn harvest). Methane efficiency equaled 314 and 299 LN∙kg-1 ODM, and its concentration in biogas amounted to 60.4 and 59.4% for summer and autumn biomass, respectively. The results indicated the possibility of storing and using biomass from roadside verges as a source of biogas. The primary aim of using added preservatives was to reduce the loss of organic matter during biomass storage as well as to improve the efficiency of methanogenesis.


AOAC. 2005. Official Methods of Analysis. 15th ed. Association of Official Analytical Chemists. Washington. DC. USA.
Florek, S., C. Purwin, D. Minakowski, M. Stanek, M. Trędowicz. 2004. The influence of formic acid additives on silage quality obtained from different plants material. Veterinary Medicine and Zootechnics Kaunas 26, 48: 23-28.
Gołaszewski, J. 2011. The use of agricultural substrates in Polish biogas plants. Postępy Nauk Rolniczych 2: 69-94 (in Polish).
Massė, D., Y. Gibert, P. Savoie, G. Bėlanger, G. Parent, D. Babineau. 2011. Methane yield from switchgrass and reed canarygrass grown in Eastern Canada. Bioresource Technology 102: 10286-10292.
McEniry, J., P. O'Kiely. 2012. Grass for biogas – the effect of advancing plant maturity and ensiling on methane production. XVI International Silage Conference. Hämeenlinna, Finland, 2-4 July 2012, pp. 458-459.
Mikołajczak, J., B. Wróbel, A. Jurkowski. 2009. Possibilities and limitations in biogas production from permanent grassland biomass in Poland. Woda-Środowisko-Obszary Wiejskie 2: 139-155 (in Polish).
Pieńkowski, C.A. 2010. The possibilities of using renewable sources of energy in Podlaskie Province. Polish Journal of Environmental Studies 19: 537-544.
Podlaski, S., D. Chołuj, G. Wiśniewski. 2010. Production of biomass from energy crops. Postępy Nauk Rolniczych 2: 163-174 (in Polish).
Pakarinen, O., A. Lehtomaki, S. Rissanen, J. Rintala. 2008. Storing energy crops for methane ptoduction: effect of solid content and biological additive. Bioresource Technology 99: 7074-7082.
Plöchl, M., H. Zacharias, C. Herrmann, M. Heiermann, A. Prochnow. 2009. Influence of silage additives on methane yield and economic performance of selected feedstock. Agricultural Engineering International: The CIGR Ejournal. Manuscript 1123. Vol. XI. June 2009.
Prochnow, A., M. Heiermann, C. Idler, B. Linke, M. Pöchl, T. Amon, H. Langeveld, P. J. Hobss. 2008. Biogas yields from grassland. Grassland Sciences European 13: 727-729.
Stelmach, E., J. Stelmach, L. Krzystek, S. Ledakowicz. 2010. Influence of carbon and nitrogen content on biogas production rate from organic fraction of municipal solid waste. Inżynieria i Aparatura Chemiczna 49: 107-108 (in Polish).
Thomas, T.A. 1977. An automated procedure for the determination of soluble carbohydrates in herbage. Journal of the Science of Food and Agriculture 28: 639-642.
Van Soest, P.J., J.B. Robertson, B.A. Lewis. 1991. Methods of dietary fiber, neutral detergent fiber and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74: 3583-3597.

  © ChemProf 2009